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Ubiquitous in living organisms, it occurs naturally in plants, fungi and animal cells

The cycle threshold (CT) for the target genes ( SIRT-1 and CYP2E1 ) and the endogenous control GAPDH for each sample were detected and the relative quantitation of SIRT-1 and CYP2E1 mRNA expression levels was estimated by normalizing CT of each gene with the CT of GAPDH in each sample using the 2 CT method (Livak and Schmittgen, 2001)

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Obese subjects respond to the stimulatory effect of the ghrelin agonist growth hormone-releasing peptide-2 on food intake