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Mutation of this residue to alanine resulted in complete loss of activity consistent with a role for Arginine 130 in stabilizing the strongly nucleophilic GSH thiolate required for catalysis (Table 1)
Enzymatic activity also results in GSSG (oxidized glutathione), as byproduct of antioxidant activity, returning to its glutathione reduced form
Both drugs showed similar inhibition efficiency on COX activity, but the naproxen group experienced more ulcers, particularly larger ones, compared to the ATB-346 group
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