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Study Objective in the Peptide Mapping Study of Semaglutide The goal of this Peptide Mapping Study of Semaglutide was to develop a high-resolution analytical workflow capable of: Characterizing the complete primary structure of semaglutide Confirming the location of its lipid modification Achieving full sequence coverage using complementary enzymatic digestion Demonstrating structural equivalence between synthetic semaglutide and the rDNA-derived reference product Developing this type of workflow is essential for peptide generic development because regulatory authorities require strong proof of structural identity

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