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Following blocking, cells were stained with pre-conjugated FAK 4.47 Ab- Alexa Fluor 555 (Millipore Sigma, 16-234) diluted 1:500 in Antibody Diluent Reagent Solution (Invitrogen, 003218) and Paxillin B2 Ab-Alex Fluor 647 (Santa Cruz Biotechnology, sc-365379 AF647) diluted 1:200 in Antibody Diluent Reagent Solution (Invitrogen, 003218) in the dark overnight at 4 C