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Levels of TAC (mM/L) were measured photometrically using a commercial kit (Bio-diagnostics, Giza, Egypt) via a spectrophotometer adjusted at a wavelength of 505 nm and following the manufacturers instruction with an intra-assay coefficient of 3.4% and at 0.04 mM/L as a least detectable concentration of TAC [27]
in 2023, the most recent year for which data are available
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