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The phases of the estrous cycle were identified using vaginal cytology: a predominance of nucleated epithelial cells indicated the proestrus stage, the presence of mostly cornified squamous epithelial cells signified the estrus stage, a mix of cornified squamous epithelial cells and leukocytes characterized the metestrus stage, and a predominance of leukocytes marked the diestrus stage ( 2.3 Morphological evaluation of ovaries The mice were euthanized and the ovaries were carefully dissected, fixed in 4% paraformaldehyde at 4C overnight, and then embedded in paraffin blocks
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Wipe the rubber stopper on the vial with an alcohol swab and allow it to dry completely
Alpha lipoic acid is a glutathione replenishing disulfide that increases whole blood and intracellular GSH levels
