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Another important limitation is receptor recycling and target re-emergence
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Statistical significance between pairs of groups was assessed using an unpaired two-sided t-test, one-way ANOVA followed by the Dunnett multiple comparison test, or two-way ANOVA with the Tukey multiple comparison test, as indicated
These limitations do not invalidate preclinical findings but emphasize the need for human clinical trials with appropriate sample sizes, standardized protocols, and validated outcome measures before establishing clinical efficacy in human patients
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