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*Please note that the linearity of the calibration curve will not be obtained if the coloration time is prolonged to enhance the coloration

Unlike the C-terminal amide of Melanotan II, the free carboxylate here is titratable and carries an additional negative charge at neutral pH, so record buffer pH alongside concentration when comparing the two molecules in the same assay

Preclinical literature frequently references cellular and animal models involving endocrine regulation, metabolic signaling, and hormone secretion dynamics when examining these peptides individually or in combination