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At the respective analysis point, the medium was removed, washed with 200 l PBS and replenished with 200 l fresh medium per well

study (1998) compared four topical treatments on human skin over one month, with immunohistological analysis of skin biopsies (actual tissue samples examined under a microscope)

Its important to keep in mind that results vary

J Med Chem 27:591596 Nakagawa T, Ito T, Fujimura S, Chikui M, Mizumura T, Miyaji T, Yurimoto H, Kato N, Sakai Y, Tomizuka N (2004) Molecular characterization of glutathione-dependent formaldehyde dehydrogenase gene FLD1 from methylotrophic yeast Pichia methanolica