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Brains were then snap-frozen in isopentane and the OB was cut into 18 m-thick sections afterwards, using a cryostat (Leica Biosystems)
Retrieved 23 January 2018
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The study also found that 99.2% of patients achieved at least 5% weight loss, 93.9% lost 10% or more, and nearly half (47.8%) achieved 20% or more body weight loss
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