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reaction Mix Dilution table 1/2 Plate one Plate two Plates four Plates nADPh Concentrate 140 L 260 L 500 L 1 mL Glutathione reductase Concentrate 140 L 260 L 500 L 1 mL Assay Buffer 1.12 mL 2.08 mL 4 mL 8 mL total reaction Mix Volume 1.4 mL 2.6 mL 5 mL 10 mL www.ArborAssays.com 9 Standard Preparation for the measurement of oxidized Glutathione (GSSG), a 50 L aliquot of the 250 M Oxidized Glutathione Standard should be treated with 1 L of 2VP as outlined on page 9
Bifunctional enzymes can be advantageous in many industrial applications including the removal of toxic chemicals and/or chemoprotective agent activity especially when the oxidase activity is enhanced by directed evolution or engineering
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