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Immune cells use ROS to sustain their functions and need adequate levels of antioxidant defenses to avoid harmful effects caused by excessive ROS production, since accurate balance between ROS and intracellular antioxidants is essential for a normal function of the cell (Banjac et al., 2008
It is likewise important to avoid multiple freeze-thaw cycles as this leads to cumulative damage
In addition, sulforaphane rescued rotenone-inhibited autophagy, as detected by LC3-II
10.1016/0140-6736(91)93130-2 Lancet 235 WeekleyC
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