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& Nemmani, K
Membranes were incubated with primary antibody overnight diluted in TBS-T/milk (or TBS-T/BSA for anti-phospho antibodies) at 4 C, washed in TBS-T and incubated for 1 hour at RT with the corresponding HRP-conjugated secondary antibody in TBS-T/milk
Ipamorelin (Aib-His-D-2-Nal-D-Phe-Lys-NH2) is a five-amino-acid peptide
In vitro human plasma stability Human plasma was preheated at 37 C for 15 min before being spiked with a final concentration of 0.25 mM of the peptide or conjugate
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